DNA Nanoclusters Combined with One‐Shot Radiotherapy Augment Cancer Immunotherapy Efficiency

• DOI: 10.1002/adma.202208546
• Publication Date: 2023-02-06T18:15:18Z
• Authors: Yuexia Xie; Huishan Li; Lei Xu; Hanbing Zou; Xingang Wang; Xiaozhen He; Qianyun Tang; Yan Zhou; Xue Zhao; Xiaojing Chen; Hongmei Liu; Jun Pu; Dan Luo; Peifeng Liu
• Keywords: Augment; Nanoclusters; Cancer Immunotherapy

Abstract Immunotherapy shows immense promise for improving cancer treatment. Combining immunotherapy with radiotherapy provides a conspicuous advantage due to its enhanced abscopal effect. However, established immune tolerance mechanisms in the tumor microenvironment can hamper generation of sufficient Herein, type DNA nanocluster (DNAnc) that is self‐assembled by CpG‐ODNs‐loaded Y‐shaped double‐stranded vector based on unique complementary base‐pairing rules designed. The structure DNAnc makes it load more than ≈8125.5 ± 822.5 copies CpG ODNs within one single nanostructure, which effectively increases resistance nuclease degradation and elevates efficiency repolarizing macrophages an M1‐like phenotype. Mechanistic studies reveal DNAncs are endocytosed tissue repolarized elicit robust effect accumulation induced radiotherapy, generating potent, long‐term, durable antitumor immunity inhibition metastasis prevention recurrence, novel strategy boost immunotherapy.