A Single‐Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

• DOI: 10.1002/adma.202304102
• Publication Date: 2023-07-15T11:10:37Z
• Authors: Enrico Genco; Francesco Modena; Lucia Sarcina; Kim Björkström; Celestino Brunetti; Mario Caironi; Mariapia Caputo; Virginia Maria De...; Cinzia Di Franco; Giulia Frusconi; Lena Haeberle; Piero Larizza; Maria Teresa Mancini; Ronald Österbacka; William Reeves; Gaetano Scamarcio; Cecilia Scandurra; May Wheeler; Eugenio Cantatore; Irene Esposito; Eleonora Macchia; Fabrizio Torricelli; Fabrizio Antonio ...; Luisa Torsi
• Keywords: singlemolecules; bioelectronics; biomarkers; early detection; pancreatic cancer precursors; printed-electronics; single molecules; single-molecule-with-a-large-transistor; pancreatic cancer precursors; Cysts; 610; biomarkers; printed-electronics; bioelectronics; SDG 3 – Goede gezondheid en welzijn; pancreatic cancer precur-sors; 3. Good health; Proto-Oncogene Proteins p21(ras)/genetics; Proto-Oncogene Proteins p21(ras); Pancreatic Neoplasms; single-molecule-with-a-large-transistor; SDG 3 - Good Health and Well-being; Humans; Pancreatic Neoplasms/diagnosis; single molecules; early detection; Early Detection of Cancer

Abstract A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96‐well bioelectronic sensing‐array single‐molecule assay in cysts fluid and blood plasma, deployable at point‐of‐care (POC). Pancreatic are mucinous diagnosed sensitivity most 80% by state‐of‐the‐art cytopathological molecular analyses (e.g., KRAS mut DNA). Adding the simultaneous proteins related to malignant transformation MUC1 CD55) deemed essential enhance diagnostic accuracy. The array proposed here, based on single‐molecule‐with‐a‐large‐transistor (SiMoT) technology, can both nucleic acids limit‐of‐identification (LOI) (1% false‐positives false‐negatives). It comprises an enzyme‐linked immunosorbent (ELISA)‐like 8 × 12‐array organic‐electronics disposable cartridge electrolyte‐gated organic transistor sensor array, reusable reader, integrating custom Si‐IC chip, operating via software installed USB‐connected smart device. complemented 3D‐printed sensing gate cover plate. , MUC1, CD55 biomarkers either plasma or cysts‐fluid from 5 6 time, multiplexed LOI 1.5 h. classified machine‐learning analysis resulting least 96% diagnostic‐sensitivity 100% diagnostic‐specificity. This preliminary study opens way POC liquid‐biopsy‐based early diagnosis pancreatic‐cancer plasma.