Direct 3D‐Bioprinting of hiPSC‐Derived Cardiomyocytes to Generate Functional Cardiac Tissues

• DOI: 10.1002/adma.202305911
• Publication Date: 2023-09-01T11:00:17Z
• Authors: Tilman U. Esser; Annalise Anspach; Katrin A. Muenzeb...; Delf Kah; Stefan Schrüfer; Joachim Schenk; Katrin G. Heinze; Dirk W. Schubert; Ben Fabry; Felix B. Engel
• Keywords: 3D bioprinting

3D-bioprinting is a promising technology to produce human tissues as drug screening tool or for organ repair. However, direct printing of living cells has proven difficult. Here, method presented directly 3D-bioprint induced pluripotent stem cell (hiPSC)-derived cardiomyocytes embedded in collagen-hyaluronic acid ink, generating centimeter-sized functional ring- and ventricle-shaped cardiac an accurate reproducible manner. The printed contain hiPSC-derived with well-organized sarcomeres exhibit spontaneous regular contractions, which persist several months are able contract against passive resistance. Importantly, beating frequencies the can be modulated by pharmacological stimulation. This approach opens up new possibilities complex models advanced tissue grafts repair replacement.