Engineering Mesoscale T Cell Receptor Clustering by Plug‐and‐Play Nanotools

• DOI: 10.1002/adma.202310407
• Publication Date: 2024-06-26T02:40:25Z
• Authors: M. Florencia Sánchez; Sevi Faria; Stefan Frühschulz; Lars Werkmann; Christian Winter; Tina Karimian; Peter Lanzerstorfer; Birgit Plochberger; Julian Weghuber; Robert Tampé
• Keywords: Immunological synapse

T cell receptor (TCR) clustering and formation of an immune synapse are crucial for TCR signaling. However, limited information is available about these dynamic assemblies their connection to transmembrane In this work, controlled via plug-and-play nanotools based on engineered irreversible conjugation pair a peptide-loaded major histocompatibility complex (pMHC) molecule compare assembly in ligand (pMHC)-induced or ligand-independent manner. A streptavidin-binding peptide displayed both tools enabled anchoring streptavidin-pre-structured matrices. Strikingly, pMHC-induced the confined regions exhibit higher density dynamics than ligand-free approach, indicating that size architecture pMHC influences assembly. This approach enables control membrane with high specificity provides possibility explore different modalities activation.