Multifunctional Conductive and Electrogenic Hydrogel Repaired Spinal Cord Injury via Immunoregulation and Enhancement of Neuronal Differentiation

• DOI: 10.1002/adma.202313672
• Publication Date: 2024-02-03T04:38:25Z
• Authors: Mingshan Liu; Wencan Zhang; Shuwei Han; Dapeng Zhang; Xiaolong Zhou; Xianzheng Guo; Haosheng Chen; Haifeng Wang; Lin Jin; Shiqing Feng; Zhijian Wei
• Keywords: Neurons; Mice; 0303 health sciences; 03 medical and health sciences; Magnetic Fields; Neural Stem Cells; Electric Conductivity; Animals; Hydrogels; Cell Differentiation; Spinal Cord Injuries

Abstract Spinal cord injury (SCI) is a refractory neurological disorder. Due to the complex pathological processes, especially secondary inflammatory cascade and lack of intrinsic regenerative capacity, it difficult recover function after SCI. Meanwhile, simulating conductive microenvironment spinal reconstructs electrical neural signal transmission interrupted by SCI facilitates repair. Therefore, double‐crosslinked hydrogel (BP@Hydrogel) containing black phosphorus nanoplates (BP) synthesized. When placed in rotating magnetic field (RMF), BP@Hydrogel can generate stable signals exhibit electrogenic characteristic. In vitro, shows satisfactory biocompatibility alleviate activation microglia. RMF, enhances anti‐inflammatory effects. wireless stimulation promotes differentiation stem cells (NSCs) into neurons, which associated with PI3K/AKT pathway. vivo, injectable elicit behavioral electrophysiological recovery complete transected mice alleviating inflammation facilitating endogenous NSCs form functional neurons synapses under RMF. The present research develops multifunctional for repair targeting multiple mechanisms including immunoregulation enhancement neuronal differentiation.