Matrix Viscoelasticity Controls Differentiation of Human Blood Vessel Organoids into Arterioles and Promotes Neovascularization in Myocardial Infarction
Organoid
Regenerative Medicine
DOI:
10.1002/adma.202410802
Publication Date:
2024-12-17T08:37:43Z
AUTHORS (15)
ABSTRACT
Stem cell-derived blood vessel organoids are embedded in extracellular matrices to stimulate sprouting. Although vascular 3D collagen I-Matrigel gels currently available, they primarily capillaries composed of endothelial cells (ECs), pericytes, and mesenchymal stem-like cells, which necessitate mature arteriole differentiation for neovascularization. In this context, the hypothesis that matrix viscoelasticity regulates development is investigated cultures by encapsulating within viscoelastic gelatin/β-CD assembly dynamic hydrogels or methacryloyl gelatin non-dynamic hydrogels. The hydrogel demonstrate enhanced angiogenesis into arterioles containing smooth muscle cells. mechanical microenvironment promotes patterning arteriolar elevating notch receptor 3 signaling stem downregulating platelet-derived growth factor B expression ECs. Transplantation vivo, along with hydrogel, leads reassembly restoration cardiac function infarcted hearts. These findings indicate properties play a crucial role controlling organization processes, suggesting an exciting potential its application regenerative medicine.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (46)
CITATIONS (2)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....