Personalized Vascularized Tumor Organoid‐on‐a‐Chip for Tumor Metastasis and Therapeutic Targeting Assessment

• DOI: 10.1002/adma.202412815
• Publication Date: 2024-12-27T04:47:07Z
• Authors: Yang Du; Yi‐Ran Wang; Qi‐Yuan Bao; Xin‐Xin Xu; Congling Xu; Shaoxuan Wang; Qi Liu; Fan Liu; Yu‐Lian Zeng; Ya‐Jun Wang; Wei Liu; Yixin Liu; Sai‐Xi Yu; Yu‐Chen Chen; Chen Wang; Weibin Zhang; Hai Gao; Hao Luo; Baohong Liu; Guangyin Jing; Ming Guo; Fei Xavier Chen; Yan‐Jun Liu
• Keywords: Organoid; Circulating tumor cell; Personalized Medicine

While tumor organoids have revolutionized cancer research by recapitulating the cellular architecture and behaviors of real tumors in vitro, their lack functional vasculature hinders attainment full physiological capabilities. Current efforts to vascularize are struggling achieve well-defined vascular networks, mimicking intricate hierarchy observed vivo, which restricts relevance particularly for studying progression response therapies targeting vasculature. An innovative vascularized patient-derived (PDTOs)-on-a-chip with hierarchical, tumor-specific microvasculature is presented, providing a versatile platform explore tumor-vascular dynamics antivascular drug efficacy. It found that highly metastatic cells induced vessel angiogenesis simultaneously migrated toward blood vessels via Notch pathway. The evident association between angiogenic migratory capacities PDTOs clinical outcomes underscores potential evaluating metastasis, thus offering valuable insights decision-making. Ultimately, system represents promising avenue advancing understanding metastasis developing personalized treatment strategies based on patient-specific characteristics.