Bioactive Coatings on 3D Printed Polycaprolactone Scaffolds for Bone Regeneration: A Novel Murine Femur Defect Model for Examination of the Biomaterial Capacity for Repair

• DOI: 10.1002/admi.202400389
• Publication Date: 2024-11-22T06:34:14Z
• Authors: Karen M. Marshall; Jonathan P. Wojci...; Vineetha Jayawarna; Abshar Hasan; Cécile Echalier; Sebastien J. P. C...; Tao Yang; Janos M. Kanczler; Jonathan I. Dawson; Alvaro Mata; Manuel Salmeron‐S...; Molly M. Stevens; Richard O. C. Oreffo
• Keywords: Polycaprolactone; Biomaterial; 3d printed; Biocompatible material

Abstract Bone tissue engineering seeks to develop treatment approaches for nonhealing and large bone defects. An ideal biodegradable scaffold will induce support formation. The current study examines augmentation in critical‐sized defects, using functionalized scaffolds, with the hypothesized potential skeletal cell differentiation. 3D printed, porous poly(caprolactone) trimethacrylate (PCL‐TMA900) scaffolds are applied within a murine femur defect, stabilized by polyimide intramedullary (IM) pin. PCL‐TMA900 coated i) elastin‐like polypeptide (ELP), ii) poly(ethyl acrylate) (PEA)/fibronectin (FN)/bone morphogenetic protein‐2 (PEA/FN/BMP‐2), iii) both ELP PEA/FN/BMP‐2, or iv) Laponite nanoclay binding BMP‐2. Sequential microcomputed tomography (µCT) histological analysis performed. is robust biocompatible when material BMP‐2 consistent, significant formation compared uncoated scaffold. Critically, retained, due Laponite, producing around desired shape volume, observed positive control (collagen sponge/BMP‐2). and/or PEA/FN/BMP‐2 do not demonstrate consistent In summary, Laponite/BMP‐2 offer biodegradable, osteogenic construct development into scale polymer clinical translation.