Microneedles with Interdigitated Electrodes for In Situ Impedimetric VEGF Sensing

• DOI: 10.1002/admi.202400789
• Publication Date: 2025-01-09T08:48:13Z
• Authors: Ritu Das; Emin Istif; Rumeysa Cebecioglu; Mohsin Ali; Yasin Atik; Çağdaş Dağ; Eda Celikbas; Gozde Demirci; FundaYagcı Acar; Murat Hasanreisoğlu; Levent Beker

Abstract Continuous monitoring of protein biomarkers in interstitial fluid (ISF) is essential for improving patient care and outcomes. This study presents a novel electrochemical impedance spectroscopy (EIS) sensor utilizing microneedles (MNs) patterned with interdigitated electrodes the detection vascular endothelial growth factor (VEGF). The MNs, are fabricated from flexible polylactide (PLA) using simple molding technique, followed by metal deposition an pattern to serve as platform EIS sensor. After functionalization anti‐VEGF antibodies, measurements conducted detect VEGF levels, demonstrating significant change response varying concentrations target biomarker. MNs can easily penetrate rat skin hand, puncturing without mechanical breakage. innovative approach enhances sensitivity specificity biomarker paves way continuous applications diagnostics preventative medicine.