Label‐Free Detection and Translocation Dynamics Study of Single‐Molecule Herceptin Using Solid‐State Nanopores

• DOI: 10.1002/admt.202200018
• Publication Date: 2022-05-31T09:46:28Z
• Authors: Rui Hu; Wenlong Lu; Guanghao Wei; Hexin Nan; Juan Li; Qing Zhao
• Keywords: Nanopore; Biomolecule

Abstract Herceptin (or trastuzumab) is an important therapeutic monoclonal antibody (mAb) used in the treatment of HER2‐positive breast cancer. Real‐time counting and characterization a fundamental step field disease‐related diagnosis therapy. Solid‐state nanopore‐based biosensors have been proved to hold great potential characterizing properties proteins at single‐molecule level for vitro diagnosis. Here, label‐free detection detailed translocation dynamics study using solid‐state nanopores are demonstrated. By constricting nanopore size close Herceptin, sensitivity temporal resolution significantly improved, allowing delicate probing structural information Herceptin. Therefore, three types events identified through nanopores, single‐level, multi‐level spike‐like events, emerged different voltages regimes, indicating unfolding kinetics under electric field. The influence high on complex biomolecules highlighted novel prospective platform provided antibodies via as miniaturized biomedical device.