Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing

• DOI: 10.1002/advs.201900513
• Publication Date: 2019-08-27T15:47:03Z
• Authors: Hyosuk Kim; Sun Young Wang; Gijung Kwak; Yoosoo Yang; Ick Chan Kwon; Sun Hwa Kim
• Keywords: Proinflammatory cytokine; Exosome; Reprogramming; Macrophage polarization

Macrophages (Mϕs) critically contribute to wound healing by coordinating inflammatory, proliferative, and angiogenic processes. A proper switch from proinflammatory M1 anti-inflammatory M2 dominant Mϕs accelerates the processes leading favorable wound-care outcomes. Herein, an exosome-guided cell reprogramming technique is proposed directly convert for effective management. The Mϕ-derived exosomes (M2-Exo) induce a complete conversion of in vitro. reprogrammed turn Arginase (M2-marker) iNOS (M1-marker) on off, respectively, exhibit distinct phenotypic functional features Mϕs. M2-Exo has not only Mϕ factors but also various cytokines growth promoting repair. After subcutaneous administration into edge, local populations are markedly decreased increased, showing successful polarization. direct at site enhancing angiogenesis, re-epithelialization, collagen deposition. phenotype switching induced possessing excellent capability innate biocompatibility can be promising therapeutic approach inflammation-associated disorders regulating balance between pro- versus