Dissolvable Polyacrylamide Beads for High‐Throughput Droplet DNA Barcoding

• DOI: 10.1002/advs.201903463
• Publication Date: 2020-02-20T12:48:38Z
• Authors: Yongcheng Wang; Ting Cao; Jina Ko; Yinan Shen; Will Zong; Kuanwei Sheng; Wenjian Cao; Sijie Sun; Liheng Cai; Ying‐Lin Zhou; Xin‐Xiang Zhang; Chenghang Zong; Ralph Weissleder; David Weitz
• Keywords: Polyacrylamide; Limiting; Bead; Dithiothreitol

Abstract Droplet‐based single cell sequencing technologies, such as inDrop, Drop‐seq, and 10X Genomics, are catalyzing a revolution in the understanding of biology. Barcoding beads key components for these technologies. What is limiting today barcoding that easy to fabricate, can efficiently deliver primers into drops, thus achieve high detection efficiency. Here, this work reports an approach fabricate dissolvable polyacrylamide beads, by crosslinking acrylamide with disulfide bridges be cleaved dithiothreitol. The rapidly dissolved drops release DNA barcode primers. synthesize, primer cost significantly lower than previous beads. Furthermore, loaded >95% loading efficiency bead per drop dissolution does not influence reverse transcription or polymerase chain reaction (PCR) drops. Based on approach, used RNA protein analysis.