Silver Covalently Bound to Cyanographene Overcomes Bacterial Resistance to Silver Nanoparticles and Antibiotics

• DOI: 10.1002/advs.202003090
• Publication Date: 2021-05-03T12:07:54Z
• Authors: David Panáček; Lucie Hochvaldová; Aristides Bakandr...; Tomáš Malina; Michal Langer; Jan Belza; Jana Martincová; Renata Večeřová; Petr Lazar; Kateřina Poláková; Jan Kolařík; Lucie Válková; Milan Kolář; Michal Otyepka; Aleš Panáček; Radek Zbořil
• Keywords: Silver nanoparticle; Flagellin

The ability of bacteria to develop resistance antibiotics is threatening one the pillars modern medicine. It was recently understood that can even silver nanoparticles by starting produce flagellin, a protein which induces their aggregation and deactivation. This study shows covalently bound cyanographene (GCN/Ag) kills silver-nanoparticle-resistant at concentrations 30 times lower than nanoparticles, challenge has been so far unmet. Tested also against multidrug resistant strains, antibacterial activity GCN/Ag systematically found as potent free ionic or 10 nm colloidal nanoparticles. Owing strong multiple dative bonds between nitrile groups silver, theory experiments confirm, there marginal ion leaching, after six months storage, thus very high cytocompatibility human cells. Molecular dynamics simulations suggest interaction with bacterial membrane, corroborated experiments, does not rely on release ions. Endowed these properties, rigid supports selectively densely functionalized silver-binding ligands, such cyanographene, may open new avenues microbial resistance.