Pten Regulates Cardiomyocyte Differentiation by Modulating Non‐CG Methylation via Dnmt3

• DOI: 10.1002/advs.202100849
• Publication Date: 2021-07-11T13:39:51Z
• Authors: Wuming Wang; Gang Lu; Hong‐Bin Liu; Zhiqiang Xiong; Ho‐Duen Leung; Ruican Cao; Alan Lap‐Yin Pang; Xianwei Su; Patrick Wai Nok Law; Zhiju Zhao; Zi‐Jiang Chen; Wai‐Yee Chan
• Keywords: DNMT3B; Conditional gene knockout; Embryoid body

Abstract The regulation of cardiomyocyte differentiation is a fundamental aspect cardiac development and regenerative medicine. PTEN plays important roles during embryonic development. However, its role in remains unknown. In this study, low‐cost protocol for from mouse stem cells (ESCs) presented it shown that Pten deletion potently suppresses differentiation. Transcriptome analysis shows the expression series marker genes downregulated −/− cardiomyocytes. ablation induces Dnmt3b via AKT/FoxO3a pathway regulates imprinted genes, including Igf2 . Double knockout Dnmt3l rescues deficiency ESCs. DNA methylomes wild‐type embryoid bodies cardiomyocytes are analyzed by whole‐genome bisulfite sequencing. significantly promotes non‐CG (CHG CHH) methylation levels genomic differentiation, increased or Igf1r also through MAPK/ERK signaling pathway, IGF2 supplementation partially Finally, conditional mice generated verified vivo.