In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy

• DOI: 10.1002/advs.202200064
• Publication Date: 2022-06-25T02:44:36Z
• Authors: Jina Ko; Kilean Lucas; Rainer Kohler; Elias A. Halabi; Martin Wilkovitsch; Jonathan C. T. Ca...; Ralph Weissleder
• Keywords: Bioorthogonal Chemistry; Intravital microscopy; Ex vivo

Abstract The ability to observe cells in live organisms is essential for understanding their function complex vivo milieus. A major challenge today has been the limited perform higher multiplexing beyond four six colors define cell subtypes vivo. Here, a click chemistry‐based strategy presented multiplexed imaging mouse models. method uses scission‐accelerated fluorophore exchange (SAFE), which exploits highly efficient bioorthogonal mechanism completely remove fluorescent signal from antibody‐labeled It shown that SAFE‐intravital microscopy allows 1) staining of specific types dorsal and cranial window chambers mice, 2) complete un‐staining minutes, 3) chemistries at lower (µ m ) thus non‐toxic concentrations, 4) cyclic imaging. potential utility demonstrated by 12 color immune mice.