Aqueous Two‐Phase Enabled Low Viscosity 3D (LoV3D) Bioprinting of Living Matter

• DOI: 10.1002/advs.202204609
• Publication Date: 2022-12-31T03:24:01Z
• Authors: Malin Becker; Melvin Gurian; Maik Schot; Jeroen Leijten
• Keywords: 3D bioprinting; Soft matter

Abstract Embedded 3D bioprinting has great value for the freeform fabrication of living matter. However, embedded is currently limited to highly viscous liquid baths or liquid‐like solid baths. In contrast, prior crosslinking, most hydrogels are formulated as low‐viscosity solutions and therefore not directly compatible with due low shape fidelity poor print stability. The authors here present a method enable ink (LoV3D) bioprinting, based on aqueous two‐phase stabilization ink‐bath interface. LoV3D allows printing constructs at high extrusion speeds (up 1.8 m s −1 ) viability its exceedingly low‐viscosity. Moreover, liquid/liquid interfaces offer unique advantages fusing printed structures, creating intricate vasculature, modifying surfaces higher efficiencies than traditional systems. Furthermore, interfacial tension offers unprecedented nozzle‐independent control over filament diameter via large‐dimension strand‐thinning, which an exceptionally wide range diameters down width single cell. Overall, all‐aqueous approach broad material compatibility without need rheological adaption, opens new avenues application in cell patterning, drug screening, engineered meat, organ fabrication.