SEMPAI: a Self‐Enhancing Multi‐Photon Artificial Intelligence for Prior‐Informed Assessment of Muscle Function and Pathology

• DOI: 10.1002/advs.202206319
• Publication Date: 2023-08-19T04:39:42Z
• Authors: Alexander Mühlberg; Paul Ritter; Simon Langer; Chloë Goossens; Stefanie Nübler; Dominik Schneidereit; Oliver Taubmann; Felix Denzinger; Dominik Nörenberg; Michael Haug; Sebastian Schürmann; Roarke Horstmeyer; Andreas K. Maier; Wolfgang H. Goldmann; Oliver Friedrich; Lucas Kreiss
• Keywords: Technology; Chemistry, Multidisciplinary; Science; Materials Science; MODELS; Materials Science, Multidisciplinary; BIOMECHATRONICS; CLASSIFICATION; meta-learning; Deep Learning; Artificial Intelligence; ALGORITHM; Nanoscience & Nanotechnology; ULTRASOUND; Research Articles; muscle research; Science & Technology; scientific machine learning; explainable artificial intelligence; Muscles; Q; deep learning; MICROSCOPY; BIOMECHANICS; CANCER; meta‐learning; prior information integration; Chemistry; PATTERN; Physical Sciences; multiphoton microscopy; Science & Technology - Other Topics; SKELETAL-MUSCLE; Neural Networks, Computer; Algorithms

AbstractDeep learning (DL) shows notable success in biomedical studies. However, most DL algorithms work as black boxes, exclude biomedical experts, and need extensive data. This is especially problematic for fundamental research in the laboratory, where often only small and sparse data are available and the objective is knowledge discovery rather than automation. Furthermore, basic research is usually hypothesis‐driven and extensive prior knowledge (priors) exists. To address this, the Self‐Enhancing Multi‐Photon Artificial Intelligence (SEMPAI) that is designed for multiphoton microscopy (MPM)‐based laboratory research is presented. It utilizes meta‐learning to optimize prior (and hypothesis) integration, data representation, and neural network architecture simultaneously. By this, the method allows hypothesis testing with DL and provides interpretable feedback about the origin of biological information in 3D images. SEMPAI performs multi‐task learning of several related tasks to enable prediction for small datasets. SEMPAI is applied on an extensive MPM database of single muscle fibers from a decade of experiments, resulting in the largest joint analysis of pathologies and function for single muscle fibers to date. It outperforms state‐of‐the‐art biomarkers in six of seven prediction tasks, including those with scarce data. SEMPAI's DL models with integrated priors are superior to those without priors and to prior‐only approaches.