Integrated Human Skin Bacteria Genome Catalog Reveals Extensive Unexplored Habitat‐Specific Microbiome Diversity and Function

• DOI: 10.1002/advs.202300050
• Publication Date: 2023-08-07T14:43:27Z
• Authors: Zhiming Li; Yanmei Ju; Jingjing Xia; Zhe Zhang; Hefu Zhen; Xin Tong; Yuzhe Sun; Haorong Lu; Yang Zong; Peishan Chen; Kaiye Cai; Zhen Wang; Huanming Yang; Jiucun Wang; Jian Wang; Yong Hou; Xin Jin; Tao Zhang; Wenwei Zhang; Xun Xu; Liang Xiao; Ruijin Guo; Chao Nie
• Keywords: Shotgun

The skin is the largest organ in human body. Various environments on its surface constitutes a complex ecosystem. One of characteristics micro-ecosystem low biomass, which greatly limits comprehensive identification microbial species through sequencing. In this study, deep-shotgun sequencing (average 21.5 Gigabyte (Gb)) from 450 facial samples and publicly available metagenomic datasets 2069 to assemble Unified Human Skin Genome (UHSG) catalog integrated. UHSG encompasses 813 prokaryotic derived 5779 metagenome-assembled genomes, among 470 are novel covering 20 phyla with 1385 assembled genomes. Based UHSG, core functions microbiome described differences amino acid metabolism, carbohydrate drug resistance different identified. Furthermore, analysis secondary metabolites near-complete genomes further find 1220 putative metabolites, several found previously unknown Single nucleotide variant (SNV) reveals possible protection mechanism: negative selection process environment conditional pathogens. offers convenient reference database that will facilitate more in-depth understanding role microorganisms skin.