Nanozyme‐Catalyzed Metasurface Plasmon Sensor‐Based Portable Ultrasensitive Optical Quantification Platform for Cancer Biomarker Screening

• DOI: 10.1002/advs.202301658
• Publication Date: 2023-06-26T13:10:46Z
• Authors: Rui Li; Hongli Fan; Hanlin Zhou; Youqian Chen; Qingcai Yu; Wenjun Hu; Gang L. Liu; Liping Huang
• Keywords: Optical sensing

Abstract Developing plasmonic biosensors that are low‐cost, portable, and relatively simple to operate remains challenging. Herein, a novel metasurface plasmon‐etch immunosensor is described, namely nanozyme‐linked immunosorbent surface plasmon resonance biosensor, for the ultrasensitive specific detection of cancer biomarkers. Gold‐silver composite nano cup array chip artificial nanozyme‐labeled antibody used in two‐way sandwich analyte detection. Changes biosensor's absorption spectrum measured before after etching, which can be applied immunoassays without requiring separation or amplification. The device achieved limit alpha‐fetoprotein (AFP) < 21.74 fM, three orders magnitude lower than commercial enzyme‐linked assay kits. Additionally, carcinoembryonic antigen (CEA) carbohydrate 125 (CA125) quantitative verify universality platform. More importantly, accuracy platform verified using 60 clinical samples; compared with hospital results, biomarkers achieve high sensitivity (CEA: 95.7%; CA125: 90.9%; AFP: 86.7%) specificity 97.3%; 93.9%; 97.8%). Due its rapidity, ease use, throughput, has potential high‐throughput rapid facilitate screening early diagnostic testing biosensing.