Primate‐Specific DAZ Regulates Translation of Cell Proliferation‐Related mRNAs and is Essential for Maintenance of Spermatogonia

• DOI: 10.1002/advs.202400692
• Publication Date: 2024-05-24T04:46:13Z
• Authors: Ningjing Ou; Yuci Wang; Shuai Xu; Jiaqiang Luo; Chenwang Zhang; Yangyi Zhang; Xiaoyan Shi; Minggang Xiong; Liangyu Zhao; Zhiyong Ji; Yuxiang Zhang; Jingpeng Zhao; Haowei Bai; Ruhui Tian; Peng Li; Erlei Zhi; Yuhua Huang; Wei Chen; Ruiqi Wang; Yuxuan Jin; Dian Wang; Zheng Li; Hao Chen; Chencheng Yao
• Keywords: Male; Adult; 0301 basic medicine; spermatogenic failure; 0303 health sciences; microdeletion of AZFc; proliferation; Science; Q; RNA-Binding Proteins; Deleted in Azoospermia 1 Protein; RNA binding protein; Spermatogonia; DAZ; Humans; Animals; RNA, Messenger; Spermatogenesis; Research Article; Cell Proliferation; Azoospermia

Primate-specific DAZ (deleted in azoospermia) has evolved the azoospermia factor c (AZFc) locus on Y chromosome. Loss of is associated with patients deletion AZFc region (AZFc_del). However, molecular mechanisms spermatogenesis remain uncertain. In this study, mechanism identified, which unknown since it identified 40 years ago because lack a suitable model. Using clinical samples and cell models, shown that plays an important role loss defective proliferation c-KIT-positive spermatogonia AZFc_del. Mechanistically, knockdown significantly downregulated global translation subsequently decreased proliferation. Furthermore, interacted PABPC1 via repeat domain to regulate translation. targeted mRNAs are involved cycle phase transition. These findings indicate master translational regulator essential for maintenance spermatogonia. may result spermatogenic failure.