On‐Chip Neural Induction Boosts Neural Stem Cell Commitment: Toward a Pipeline for iPSC‐Based Therapies

• DOI: 10.1002/advs.202401859
• Publication Date: 2024-04-24T10:33:55Z
• Authors: Saumey Jain; Dimitrios Voulgaris; Surangrat Thongkorn; Rick Hesen; Alice Hägg; Mohsen Moslem; Anna Falk; Anna Herland
• Keywords: Reprogramming; Neural cell

Abstract The clinical translation of induced pluripotent stem cells (iPSCs) holds great potential for personalized therapeutics. However, one the main obstacles is that current workflow to generate iPSCs expensive, time‐consuming, and requires standardization. A simplified cost‐effective microfluidic approach presented reprogramming fibroblasts into their subsequent differentiation neural (NSCs). This method exploits microphysiological technology, providing a 100‐fold reduction in reagents ninefold number input cells. generated from show upregulation pluripotency markers downregulation fibroblast markers, on par with those reprogrammed standard well‐conditions. NSCs differentiated chips neuroectodermal ( ZIC1 , PAX6, SOX1) highlighting propensity nervous system development. Cells obtained conventional well plates are compared induction by bulk RNA sequencing. Pathway enrichment analysis chip showed cell development boosted commitment lineage initial phases induction, attributed confined environment chip. provides pipeline reprogram differentiate therapeutics compliant good manufacturing practices.