Mitochondria Transplantation to Bone Marrow Stromal Cells Promotes Angiogenesis During Bone Repair

• DOI: 10.1002/advs.202403201
• Publication Date: 2024-08-13T19:30:38Z
• Authors: Yifan Wang; Wenjing Li; Yusi Guo; Ying Huang; Yaru Guo; Jia Song; Feng Mei; Peiwen Liao; Zijian Gong; Xiaopei Chi; Xuliang Deng
• Keywords: Regenerative Medicine; Therapeutic angiogenesis

Abstract Angiogenesis is crucial for successful bone defect repair. Co‐transplanting Bone Marrow Stromal Cells (BMSCs) and Endothelial (ECs) has shown promise vascular augmentation, but it face challenges in hostile tissue microenvironments, including poor cell survival limited efficacy. In this study, the mitochondria of human BMSCs are isolated transplanted to from same batch passage number (BMSCs mito ). The significantly boosted ability ‐ECs promote angiogenesis, as assessed by vitro tube formation spheroid sprouting assays, well vivo transplantation experiments balb/c mouse SD rat models. Dll4‐Notch1 signaling pathway found play a key role ‐induced endothelial formation. with ECs cranial improves functional network formation, improve repair outcomes. These findings thus highlight that mitochondrial transplantation, acting through DLL4‐Notch1 pathway, represents promising therapeutic strategy enhancing angiogenesis improving Hence, BMSCS approach promoting offers valuable insights holds much innovative regenerative medicine therapies.