GhLPF1 Associated Network Is Involved with Cotton Lint Percentage Regulation Revealed by the Integrative Analysis of Spatial Transcriptome

• DOI: 10.1002/advs.202414175
• Publication Date: 2025-02-11T14:59:51Z
• Authors: Hongyu Wu; Luyao Wang; Shengjun Zhao; Mengtao Gao; Junfeng Cao; Yupeng Hao; Li Yu; Ting Zhao; Siyuan Wang; Jin Han; Yumeng Zhu; Yongyan Zhao; Jie Li; Ke Nie; Kening Lu; Linyun Ding; Zhiyuan Zhang; Tianzhen Zhang; Xueying Guan
• Keywords: Lint; Gene regulatory network; MYB

Abstract Cotton fibers, derived from the epidermis of ovule, provide a sustainable natural fiber source for textile industry. Traits related to yield are predominantly determined by molecular regulations in outer integument (OI) region cotton ovule. Here, we identify an R2R3 MYB transcription factor coding gene GhLPF1 within QTL‐LP‐ChrA06 locus lint percentage (LP, seed cotton) through constructing 1‐Day Post Anthesis Ovule Spatial Transcriptome Atlas. is subjected as downstream target miR828 during development. The direct genes (DDGs) biased increased expression GhLPF1‐CR , and preferentially expressed OI, so that primarily transcriptional repressor its DDGs. Population‐wide transcriptome analysis confirms variation GhLPF1‐DDGs significantly negative correlation with LP, among which type I homeobox protein‐coding GhHB6 further validated be directly GhLPF1. Given these data, it demonstrated mediates regulation network LP repressor, makes valuable functional marker fiber‐trait improvement application QTL‐LP‐ChrA06.