Granulocyte colony stimulating factor does not induce long‐term DNA instability in healthy peripheral blood stem cell donors

• DOI: 10.1002/ajh.10324
• Publication Date: 2003-04-14T10:02:38Z
• Authors: Michael Y. Shapira; Pavel Kaspler; Simcha Samuel; Shmuel Shoshan; Reuven Or
• Keywords: Peripheral Blood Stem Cell Transplantation; Blood Donors; DNA; Hematopoietic Stem Cells; Recombinant Proteins; 3. Good health; Leukocyte Count; 03 medical and health sciences; 0302 clinical medicine; Drug Stability; Granulocyte Colony-Stimulating Factor; Leukocytes; Humans; Prospective Studies

AbstractTo establish the safety of using G‐CSF in healthy PBSC donors, we prospectively determined the degree of DNA destabilization in peripheral blood WBC. Donors were treated with SC G‐CSF for 5 days. A baseline sample of peripheral blood was collected before G‐CSF treatment and consecutive samples were collected on day 5, 1 month, and 2 months after treatment. The extent of double‐stranded DNA relaxation and de novo synthesis of DNA was determined in each sample. We found that both parameters of DNA destabilization were significantly increased after 5 days of G‐CSF and returned to baseline level at 1 and 2 months. We assume that these findings suggest that rhG‐CSF administration to healthy PBSCT donors for 5 days may be a safe procedure. Am. J. Hematol. 73:33–36, 2003. © 2003 Wiley‐Liss, Inc.