Sequence of proteome profiles in preclinical and symptomatic Alzheimer's disease

• DOI: 10.1002/alz.12345
• Publication Date: 2021-04-19T17:36:42Z
• Authors: Xiaohang Li; Konstantinos C. T...; Marta J. Koper; Alicja Ronisz; Simona Ospitalieri; Christine A.F. vo...; Rik Vandenberghe; Thomas Tousseyn; Angelika Scheuerle; Anastassios Economou; Sebastien Carpentier; Markus Otto; Dietmar Rudolf Thal
• Keywords: Proteome

Abstract Proteome profile changes in Alzheimer's disease (AD) brains have been reported. However, it is unclear whether they represent a continuous process, or there sequential involvement of distinct proteins. To address this question, we used mass spectrometry. We analyzed soluble, dispersible, sodium dodecyl sulfate, and formic acid fractions neocortex homogenates (mainly Brodmann area 17‐19) from 18 pathologically diagnosed preclinical AD, 17 symptomatic cases without signs neurodegeneration. By doing so, identified four groups AD‐related proteins being changed levels AD cases: early‐responding, late‐responding, gradually‐changing, fraction‐shifting Gene ontology analysis these all known AD‐risk/causative genes vesicle endocytosis the secretory pathway‐related processes as an early‐involved component. In conclusion, our findings suggest that subtle involving pathway precede severe proteome part phase AD. The respective early‐responding may also contribute to synaptic cycle alterations