A mouse coccygeal intervertebral disc degeneration model with tail‐looping constructed using a suturing method

• DOI: 10.1002/ame2.12501
• Publication Date: 2025-01-14T15:57:00Z
• Authors: Wei Xie; Zemao Huang; Ziyang Huang; Deqing Luo; Zhangxin Chen; Li Xie; Lingqi Zhu; Hui Liu; Kejian Lian; Paolo Alberton; Denitsa Docheva; Dasheng Lin
• Keywords: Intervertebral Disc; Degeneration (medical)

Abstract Backgroud Intervertebral disc degeneration (IDD) is one of the common degenerative diseases. Due to ethical constraints, it difficult obtain sufficient research on humans, so use an animal model IDD very important clarify pathogenesis and treatment mechanism disease. Methods In this study, thirty 2‐month‐old mice were selected for operation establish a coccygeal model. The distal tail portion (beyond 17th vertebra) small piece skin above 8th vertebra excised, two incisions brought together after flexion, secured with sutures. heights signal intensities intervertebral discs (IVDs) assessed using microcomputed tomography (μCT) magnetic resonance imaging (MRI) at 0, 6, 12 weeks postoperatively. overall tissue morphology, cell distribution density, extracellular matrix IVDs also Hematoxylin Eosin (HE), Safranin O‐Fast Green immunohistochemical staining. Results All in experimental group survived operation, there no complications such as wound infection, necrosis suture shedding. results demonstrated that suturing method can successfully initiate IDD. Different severity levels be induced by controlling bending angle within loop; however, consistency, histologic should obtained same looping period. Conclusions This effective studying etiology IVD