Ephrin‐B2 prevents N‐methyl‐D‐aspartate receptor antibody effects on memory and neuroplasticity

• DOI: 10.1002/ana.24721
• Publication Date: 2016-07-12T00:07:12Z
• Authors: Jesús Planagumà; Holger Haselmann; Francesco Mannara; Mar Petit‐Pedrol; Benedikt Grünewald; Esther Aguilar; Luise Röpke; Elena Martín‐García; Maarten J. Titulaer; Pablo Jercog; Francesc Graus; Rafael Maldonado; Christian Geis; Josep Dalmau
• Keywords: Schaffer collateral

To demonstrate that ephrin-B2 (the ligand of EphB2 receptor) antagonizes the pathogenic effects patients' N-methyl-D-aspartate receptor (NMDAR) antibodies on memory and synaptic plasticity.One hundred twenty-two C57BL/6J mice infused with cerebrospinal fluid (CSF) from patients anti-NMDAR encephalitis or controls, without ephrin-B2, were investigated. CSF was through ventricular catheters connected to subcutaneous osmotic pumps over 14 days. Memory, behavioral tasks, locomotor activity, presence human specifically bound hippocampal NMDAR, antibody density cell-surface NMDAR examined at different time points using reported techniques. Short- long-term plasticity determined in acute brain sections; Schaffer collateral pathway stimulated field excitatory postsynaptic potentials recorded CA1 region hippocampus.Mice CSF, but not control developed progressive deficit depressive-like behavior along deposits hippocampus. These findings associated a decrease EphB2, marked impairment altering short-term plasticity. Administration prevented all investigated paradigms assessing memory, behavior, plasticity.Administration prevents levels reveal strategy beyond immunotherapy antagonize effects. Ann Neurol 2016;80:388-400.