Development of Multinuclear Polymeric Nanoparticles as Robust Protein Nanocarriers

• DOI: 10.1002/ange.201404766
• Publication Date: 2014-07-02T19:58:23Z
• Authors: Jun Wu; Nazila Kamaly; Jinjun Shi; Lili Zhao; Zeyu Xiao; Geoffrey Hollett; Rohit John; Shaunak Ray; Xiaoyang Xu; Xueqing Zhang; Philip W. Kantoff; Omid C. Farokhzad
• Keywords: Nanocarriers; PLGA; Nanocapsules; Surface charge

Abstract One limitation of current biodegradable polymeric nanoparticles is their inability to effectively encapsulate and sustainably release proteins while maintaining protein bioactivity. Here we report the engineering PLGA–polycation with a core–shell structure that act as robust vector for encapsulation delivery peptides. The optimized can load high amounts (>20 % by weight) in aqueous solution without organic solvents through electrostatic interactions simple mixing, thereby forming nanospheres seconds diameters <200 nm. relationship between nanosphere size, surface charge, composition, loading also investigated. stable complexes contain multiple nanoparticles, surrounded large protein. This study highlights novel strategy other relevant molecules.