In Vivo Residue‐Specific Dopa‐Incorporated Engineered Mussel Bioglue with Enhanced Adhesion and Water Resistance

• DOI: 10.1002/ange.201406099
• Publication Date: 2014-09-01T12:57:01Z
• Authors: Byeongseon Yang; Niraikulam Ayyadurai; Hyungdon Yun; Yoo Seong Choi; Byeong Hee Hwang; Jun Huang; Qingye Lu; Hongbo Zeng; Hyung Joon Cha
• Keywords: Residue (chemistry)

Abstract Misaminoacylation of 3,4‐dihydroxyphenylalanine (Dopa) molecules to tRNA Tyr by endogenous tyrosyl‐tRNA synthetase allowed the quantitative replacement tyrosine residues with a yield over 90 % an in vivo residue‐specific incorporation strategy, create, for first time, engineered mussel adhesive proteins (MAPs) Escherichia coli very high Dopa content, close that natural MAPs. The Dopa‐incorporated MAPs exhibited superior surface adhesion and water resistance ability assistance Dopa‐mediated interactions including oxidative cross‐linking, furthermore, showed underwater properties comparable those These results propose promising use as bioglues or hydrogels practical applications.