Posttranslational Modifications of Intact Proteins Detected by NMR Spectroscopy: Application to Glycosylation

• DOI: 10.1002/ange.201502093
• Publication Date: 2015-04-29T21:46:07Z
• Authors: Mario Schubert; Michal J. Walczak; Markus Aebi; Gerhard Wider
• Keywords: Isotopic labeling; Posttranslational modification

Abstract Posttranslational modifications (PTMs) are an integral part of the majority proteins. The characterization structure and function PTMs can be very challenging especially for glycans. Existing methods to analyze require complicated sample preparations suffer from missing certain modifications, inability identify linkage types thus chemical structure. We present a direct, robust, simple NMR spectroscopy method detection identification in No isotope labeling is required, nor does molecular weight studied protein limit application. directly detect on intact proteins without sophisticated preparation. This approach well suited diagnostics derived native organisms quality control biotechnologically produced therapeutic