A Ratiometric Fluorescent Probe for Imaging of the Activity of Methionine Sulfoxide Reductase A in Cells

• DOI: 10.1002/ange.201605833
• Publication Date: 2016-09-14T10:04:09Z
• Authors: Nikolai Makukhin; Vyacheslav Tretya...; Jackob Moskovitz; Jiří Míšek
• Keywords: MSRA; Methionine sulfoxide reductase; Methionine sulfoxide; Bathochromic shift; Sulfoxide

Abstract Methionine sulfoxide reductase A (MsrA) is an enzyme involved in redox balance and signaling, its aberrant activity implicated a number of diseases (for example, Alzheimer's disease cancer). Since there no simple small molecule tool to monitor MsrA real time vivo, we aimed at developing one. We have designed BODIPY‐based probe called ( S )‐Sulfox‐1, which equipped with reactive moiety. Upon reduction model E. coli ), it exhibits bathochromic shift the fluorescence maximum. This feature was utilized for real‐time ratiometric fluorescent imaging cells. Significantly, our capable capturing natural variations vivo.