A Reversible Fluorescent Probe for Real‐Time Quantitative Monitoring of Cellular Glutathione

• DOI: 10.1002/ange.201702114
• Publication Date: 2017-03-28T14:02:09Z
• Authors: Zhixue Liu; Xin Zhou; Yu Miao; Ying Hu; Nahyun Kwon; Xue Wu; Juyoung Yoon
• Keywords: HeLa; Dissociation constant

Abstract The ability to monitor and quantify glutathione (GSH) in live cells is essential order gain a detailed understanding of GSH‐related pathological events. However, owing their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed ratiometric probe (QG‐ 1 ) quantitatively monitoring cellular GSH. responds specifically reversibility with an ideal dissociation constant ( K d 2.59 m fast time t 1/2 =5.82 s). also demonstrate that QG‐ detection feasible model protein system. was found extremely low cytotoxicity applied determine the concentration HeLa (5.40±0.87 ).