Discrete Hf18 Metal‐oxo Cluster as a Heterogeneous Nanozyme for Site‐Specific Proteolysis

• DOI: 10.1002/ange.202001036
• Publication Date: 2020-03-10T10:42:45Z
• Authors: Jens Moons; Francisco de Azam...; Jelena Mihailovic; Karoly Kozma; Katarina Smiljanic; Mehran Amiri; Tanja Cirkovic Ve...; May Nyman; Tatjana N. Parac‐...
• Keywords: Random hexamer

Abstract The selective hydrolysis of proteins by non‐enzymatic catalysis is difficult to achieve, yet it crucial for applications in biotechnology and proteomics. Herein, we report that discrete hafnium metal‐oxo cluster [Hf 18 O 10 (OH) 26 (SO 4 ) 13 ⋅(H 2 O) 33 ] ( Hf ), which centred the same hexamer motif found many MOFs, acts as a heterogeneous catalyst efficient horse heart myoglobin (HHM) low buffer concentrations. Among 154 amino acids present sequence HHM, strictly cleavage at only 6 solvent accessible aspartate residues was observed. Mechanistic experiments suggest hydrolytic activity likely derived from actuation IV Lewis acidic sites Brønsted surface . X‐ray scattering ESI‐MS revealed completely insoluble these conditions, confirming HHM caused reaction solid cluster, not smaller, soluble species could leach into solution.