Neutrophil‐Selective Fluorescent Probe Development through Metabolism‐Oriented Live‐Cell Distinction

• DOI: 10.1002/ange.202108536
• Publication Date: 2021-08-20T03:14:33Z
• Authors: Min Gao; Sun Hyeok Lee; Sang Hyuk Park; Larissa Miasiro C...; Haw‐Young Kwon; Heewon Cho; Joseph Jeong; Young‐Tae Chang
• Keywords: Live cell imaging; Cellular metabolism

Abstract Human neutrophils are the most abundant leukocytes and have been considered as first line of defence in innate immune system. Selective imaging live will facilitate situ study infection or inflammation events well clinical diagnosis. However, small‐molecule‐based probes for discrimination among different granulocytes human blood yet to be reported. Herein, we report fluorescent probe NeutropG specific distinction active neutrophils. The selective staining mechanism is elucidated metabolism‐oriented live‐cell (MOLD) through lipid droplet biogenesis with help ACSL DGAT. Finally, applied accurately quantify neutrophil levels fresh samples by showing a high correlation current method.