Surface‐Based Multimeric Aptamer Generation and Bio‐Functionalization for Electrochemical Biosensing Applications

• DOI: 10.1002/ange.202402808
• Publication Date: 2024-05-20T05:54:06Z
• Authors: Seyed Vahid Hamidi; Arash Khorrami Ja...; Imman I. Hosseini; Roozbeh Siavash M...; Cesar Collazos; Qinghua Pan; Chen Liang; Sara Mahshid
• Keywords: Aptamer; Rolling circle replication; Surface Modification

Abstract Multimeric aptamers have gained more attention than their monomeric counterparts due to providing binding sites for target analytes, leading increased affinity. This work attempted engineer the surface‐based generation of multimeric by employing room temperature rolling circle amplification (RCA) technique and chemically modified primers developing a highly sensitive selective electrochemical aptasensor. The aptamers, generated through surface RCA, are hybridized spacer primers, facilitating positioning in proximity sensing surfaces. These can be used as bio‐receptors capturing specific targets. process was fully characterized, optimal time biosensing purposes determined, using SARS‐CoV‐2 spike protein (SP). Interestingly, aptasensors produced considerably higher response signals affinity (more 10‐fold), well sensitivity (almost 4‐fold) compared aptasensors. Furthermore, impact structures on studied utilizing both flat working electrodes (WEs) nano‐/microislands (NMIs) WEs. NMIs showed significantly buffer saliva media with limit detection less 2 fg/ml. Finally, developed were clinically challenged several patient samples.