miR‐TRAP: A Benchtop Chemical Biology Strategy to Identify microRNA Targets

• DOI: 10.1002/anie.201201512
• Publication Date: 2012-05-08T09:53:17Z
• Authors: Huricha Baigude; Ahsanullah; Zhonghan Li; Ying Zhou; Tariq M. Rana
• Keywords: Trap (plumbing); RNA Silencing; RNA-induced silencing complex

It′s a TRAP! microRNAs (miRNAs) are noncoding small RNAs that consist of approximately 21 nucleotides and assembled into an RNA-induced silencing complex (RISC), which suppresses target mRNA expression. An easy-to-use method called miRNA RNA affinity purification (miR-TRAP) directly identifies targets in vivo. This could be widely used to discover various disease models under physiological conditions.