Differential Sensing of MAP Kinases Using SOX‐Peptides

• DOI: 10.1002/anie.201408256
• Publication Date: 2014-10-15T15:46:42Z
• Authors: Diana Zamora‐Oliv...; Tamer S. Kaoud; Jiney Jose; Andrew Ellington; Kevin N. Dalby; Eric V. Anslyn
• Keywords: 0301 basic medicine; Sulfonamides; 03 medical and health sciences; Molecular Structure; Mitogen-Activated Protein Kinases; Peptides

AbstractFive SOX peptides are used to classify the MAPK groups and isoforms thereof using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis leads to the quantification of ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross‐reactivity among the peptides, and some unexpected surprises.