Bioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross‐Presentation

• DOI: 10.1002/anie.201500301
• Publication Date: 2015-03-17T22:42:46Z
• Authors: Joanna B. Pawlak; Geoffroy P. P. Ge...; Tracy J. Ruckwardt; Jessica S. Bremmers; Nico J. Meeuwenoord; Ferry A. Ossendorp; Herman S. Overkleeft; Dmitri V. Filippov; Sander I. van Kas...
• Keywords: Bioorthogonal Chemistry; Antigen processing; Cross-Presentation

Abstract The activation of CD8 + T‐cells requires the uptake exogenous polypeptide antigens and proteolytic processing these to octamer or nonamer peptides, which are loaded on MHC‐I complexes presented T‐cell. By using an azide as a bioorthogonal protecting group rather than ligation handle, masked were generated—antigens that not recognized by their cognate T‐cell unless they deprotected cell Staudinger reduction.