Biomimicry Promotes the Efficiency of a 10‐Step Sequential Enzymatic Reaction on Nanoparticles, Converting Glucose to Lactate

• DOI: 10.1002/anie.201609495
• Publication Date: 2016-11-30T10:37:39Z
• Authors: Chinatsu Mukai; Lizeng Gao; Jacquelyn L. Nelson; James P. Lata; Roy Cohen; Lauren Wu; Meleana M. Hinchman; Magnus Bergkvist; Robert W. Sherwood; Sheng Zhang; Alexander J. Travis
• Keywords: Nanobiotechnology

Abstract For nanobiotechnology to achieve its potential, complex organic–inorganic systems must grow utilize the sequential functions of multiple biological components. Critical challenges exist: immobilizing enzymes can block substrate‐binding sites or prohibit conformational changes, substrate composition interfere with activity, and multistep reactions risk diffusion intermediates. As a result, most tethered reaction reported involves only 3 enzymes. Inspired by oriented immobilization glycolytic on fibrous sheath mammalian sperm, here we show 10 nanoparticles. Although individual enzyme efficiency was higher in solution, efficacy 10‐step pathway measured conversion glucose lactate significantly when tethered. To our knowledge, this is system described, it shows that tethered, multi‐step pathways be reconstituted hybrid carry out such as energy production delivery molecular cargo.