Long‐Term Live‐Cell STED Nanoscopy of Primary and Cultured Cells with the Plasma Membrane HIDE Probe DiI‐SiR

• DOI: 10.1002/anie.201704783
• Publication Date: 2017-07-05T18:42:59Z
• Authors: Alexander D. Thom...; Mitchell H. Omar; Felix Rivera‐Molina; Zhiqun Xi; Anthony J. Koleske; Derek K. Toomre; Alanna Schepartz
• Keywords: STED microscopy; Temporal resolution; Live cell imaging; Filopodia

Super-resolution imaging of live cells over extended time periods with high temporal resolution requires high-density labeling and extraordinary fluorophore photostability. Herein, we achieve this goal by combining the attributes plasma membrane probe DiI-TCO photostable STED dye SiR-Tz. These components undergo rapid tetrazine ligation within to generate HIDE DiI-SiR. Using DiI-SiR, visualized filopodia dynamics in HeLa 25 min at 0.5 s resolution, dynamic contact-mediated repulsion events primary mouse hippocampal neurons 9 2 resolution. probes such as DiI-SiR are non-toxic do not require transfection, their apparent photostability significantly improves ability monitor processes super-resolution biologically relevant timescales.