A Multi‐responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super‐resolution Imaging

• DOI: 10.1002/anie.202005959
• Publication Date: 2020-06-23T03:28:56Z
• Authors: Guanqing Yang; Zhengjie Liu; Ruilong Zhang; Xiaohe Tian; Juan Chen; Guangmei Han; Bianhua Liu; Xinya Han; Yao Fu; Zhangjun Hu; Zhongping Zhang
• Keywords: 0301 basic medicine; Nuclear Proteins; Hep G2 Cells; Hydrogen Peroxide; 01 natural sciences; Mitochondria; 0104 chemical sciences; 03 medical and health sciences; Nucleic Acids; Humans; Reactive Oxygen Species; Fluorescent Dyes

AbstractUnderstanding the biomolecular interactions in a specific organelle has been a long‐standing challenge because it requires super‐resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super‐resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H2O2 level leads to disassociation of nucleic acid–protein complexes.