A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy

• DOI: 10.1002/anie.202217932
• Publication Date: 2023-01-09T17:47:29Z
• Authors: Xiaojing Wang; Skye Shepherd; Nantao Li; Congnyu Che; Tingjie Song; Yanyu Xiong; Isabella Rose Palm; Bin Zhao; Manish Kohli; Utkan Demirci; Yi Lu; Brian T. Cunningham
• Keywords: Microscopy; Photons; Biosensing Techniques; Prognosis; Exosomes; 01 natural sciences; 3. Good health; 0104 chemical sciences; MicroRNAs; Nucleic Acid Amplification Techniques

Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis-ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex with sub-attomolar sensitivity in 20 minutes, robust selectivity single nucleotide variants, a broad dynamic range from 1 aM pM. Compared traditional qRT-PCR, TRAP showed similar accuracy profiling exosomal derived cells, but also exhibited at least 31-fold 61-fold enhancement limits miRNA-375 miRNA-21 detection, respectively. The approach is ideal or circulating miRNA biomarker quantification, where are present low concentrations sample volume, potentials frequent, low-cost, minimally invasive point-of-care testing.