Antibacterial and antibiofilm activity of nanochelating based silver nanoparticles against several nosocomial pathogens

• DOI: 10.1002/aoc.4327
• Publication Date: 2018-02-28T09:18:23Z
• Authors: Seyedeh Mahsan Ho...; Abdollah Karimi; Shahnaz Armin; Saideh Fakharzadeh; Fatemeh Fallah; Somayeh Kalanaky
• Keywords: Acinetobacter baumannii; Silver nanoparticle; Broth microdilution; MTT assay

The emergence of multi‐drug resistant (MDR) bacteria and dynamic pattern infectious diseases demand to develop alternative more effective therapeutic strategies. Silver nanoparticles (AgNPs) are among the most widely commercialized engineered nanomaterials, because their unique properties increasing use for various applications in nanomedicine. This study first time aimed evaluate antibacterial antibiofilm activities newly synthesized nanochelating based AgNPs against several Gram‐positive ‐negative nosocomial pathogens. Nanochelating technology was used design synthesize AgNPs. cytotoxicity tested human cell line using MTT assay. minimal inhibitory concentration (MIC) determined by standard broth microdilution. Antibiofilm activity assayed a microtiter‐plate screening method. two including (A) with size about 20‐25 nm, (B) 30‐35 nm were Staphylococcus aureus , epidermidis Acinetobacter baumannii Pseudomonas aeruginosa . exhibited higher strains. found significantly inhibit biofilm formation strains 0.01 10 mg/mL. showed significant compared (B). In summary, our results promising new