Preparation of superparamagnetic immunomicrospheres and application for antibody purification

• DOI: 10.1002/app.21168
• Publication Date: 2004-10-01T22:01:05Z
• Authors: Xianqiao Liu; Yueping Guan; Yu Yang; Zhiya Ma; Xiaobing Wu; Huizhou Liu
• Keywords: Superparamagnetism; Glutaraldehyde; Divinylbenzene; Vinyl alcohol; Coprecipitation; Suspension polymerization; Emulsion polymerization

Abstract A novel and effective protocol for the preparation of superparamagnetic immunomicrospheres has been developed. First, micro‐size magnetic poly (methacrylate‐divinylbenzene) (PMA‐DVB) spheres were prepared by a modified suspension polymerization method. The oleic acid coated magnetite (Fe 3 O 4 ) nanoparticles made coprecipitation mixed with monomers MA, DVB, initiator benzoyl peroxide (BPO) to form oil in water emulsion droplets presence (vinyl alcohol) (PVA‐1788) as stabilizer. reaction was carried out 2‐L beaker equipped four vertical stainless steel baffleplates increasing temperature mixture at controlled rate. resulting microspheres are micro‐sized (less than 8μm diameter) 80 percent them size ranging from 1 5 μm. Then, they highly functionalized via ammonolysis ethylenediamine, surface amino‐modified obtained. morphology properties these examined SEM, TEM, VSM, FT‐IR. Affinity ligand protein (ProtA) covalently immobilized glutaraldehyde These ProtA‐immobilized affinity bioseparation processes, demonstrated efficient immunoaffinity purification antibodies IgG2a (22mg per gram microspheres) mouse ascites. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 2205–2211,