Galectin 3 and its binding protein in rheumatoid arthritis

• DOI: 10.1002/art.11287
• Publication Date: 2004-06-02T14:21:02Z
• Authors: Shiro Ohshima; Stefan Kuchen; Christian A. Seem...; Diego Kyburz; Astrid Hirt; Stephanie Klinzing; Beat A. Michel; Renate E. Gay; Fu‐Tong Liu; Steffen Gay; Michel Neidhart
• Keywords: Galectin; Galectin-1

Abstract Objective To characterize the expression pattern and role of galectin 3 binding protein (G3BP) in rheumatoid arthritis (RA), comparison with 1, to explore whether soluble G3BP, investigated serum, synovial fluid, or cell culture supernatant, are associated disease. Methods Synovial tissues from patients RA osteoarthritis (OA), as well healthy controls, were analyzed for galectins 1 G3BP by situ hybridization immunohistochemistry. Levels serum fluid OA supernatants, determined enzyme‐linked immunosorbent assay (ELISA). In vitro, intracellular fibroblasts after modulation tumor necrosis factor α (TNFα), interleukin‐1β (IL‐1β), anti‐CD40 monoclonal antibodies was measured flow cytometry. Results RA, messenger RNA stained throughout membrane, whereas particularly expressed at sites bone destruction. contrast, not uniform different specimens, never found invasion. normal tissues, only a small number cells positive and/or G3BP. Galectin elevated sera fluids, increased fluids only. correlated C‐reactive levels, joint destruction activation levels cartilage oligomeric matrix protein. showed an release into medium, ELISA, but decreased secretion low basal 3, TNFα its level dose‐dependent manner. IL‐1β no effect. Conclusion Our data indicate that involved inflammation, also contribute fibroblasts. The accumulation can be enhanced TNFα. Thus, represent novel markers disease activity RA.