Simple procedure applying lactose induction and one‐step purification for high‐yield production of rhCIFN

• DOI: 10.1002/bab.1426
• Publication Date: 2015-08-08T13:01:27Z
• Authors: Hamid Bashir; Nadeem Ahmed; Mohsin Ahmad Khan; Ahmad Usman Zafar; Saad Tahir; Muhammad Islam Khan; Faidad Khan; Tayyab Husnain
• Keywords: Inducer

Recombinant consensus interferon (CIFN) is a therapeutic protein with molecular weight of 19.5 kDa having broad spectrum antiviral activity. human CIFN (rhCIFN) has previously been expressed in Escherichia coli using isopropyl-β-d-thiogalactopyranoside (IPTG), non-metabolizable and expensive compound, as inducer. For economical commercial-scale recombinant production, it greatly needed to increase the product yield limited time frame reduce processing cost. To cost production rhCIFN E. coli, induction was accomplished by lactose instead IPTG. Lactose (14 g/L) shake flask experiment resulted higher compared 1 mM Finally, single-step purification on DEAE sepharose, 150 mg/L >98% pure achieved. In present study, an attempt made develop low process for producing quality high purity. Methods devised may be helpful pilot-scale proteins at