Retinoic acid inhibits osteogenic differentiation of mouse embryonic palate mesenchymal cells

• DOI: 10.1002/bdra.20723
• Publication Date: 2010-11-08T13:57:34Z
• Authors: Mu Chen; Hong‐Zhang Huang; Miao Wang; An‐Xun Wang
• Keywords: Tretinoin; Von Kossa stain

Abstract BACKGROUND : All‐trans‐retinoic acid (ATRA), a known teratogenic factor affecting the development of cleft palate, has been shown to adversely affect craniofacial development. In present study, we evaluated effects ATRA on osteo‐/adipogenic differentiation mouse embryonic palate mesenchymal (MEPM) cells, which served as valid model system for investigating mechanisms regulating osteogenesis during palatogenesis. METHODS MEPM cells were derived from gestational day 13 C57BL/6N embryos and induced differentiate in presence or absence either osteogenic medium (OM) control (CM). RESULTS Alkaline phosphatase (ALP) activity assays, von Kossa staining, RT‐PCR assays confirmed that underwent when cultured OM. Although ALP lipid accumulation it failed induce matrix mineralization osteoblastic gene expression. BMPR‐IB Smad5 mRNA levels increased significantly OM declined following treatment with ATRA, whereas expression BMPR‐IA was up‐regulated by ATRA. CONCLUSIONS conclusion, our results suggested BMP signaling pathway cooperate inhibit promote adipogenesis cells. Birth Defects Research (Part A), 2010. © 2010 Wiley‐Liss, Inc.