Actively cross‐linking hemostatic sealant enables rapid hemostasis and wound closure

• DOI: 10.1002/biot.202200517
• Publication Date: 2023-05-19T00:48:26Z
• Authors: Jaewook Lee; Hye Min Cho; Yoo Eun Lee; Yeonjeong Kim; Kyung Min Park; Byeong Hee Hwang
• Keywords: Biocompatibility; Hemostatic Agent

A rapid hemostatic sealant can save a patient's life from shock and death due to severe trauma or excessive bleeding the wound site during surgery. However, an ideal needs meet standards of safety, efficacy, usability, cost, approvability overcome new challenges. Here, we devised combinatorial PEG succinimidyl glutarate-based cross-linking branched polymers (CBPs) active peptide (AHP). After ex vivo optimization, best combination was called (ACHS). Interestingly, ACHS formed cross-links with serum proteins, blood cells, tissue interconnected coating on which might induce hemostasis adhesion based SEM images. Moreover, showed highest coagulation formation, agglomeration thrombi within 12 s, in vitro biocompatibility. Mouse model experiments represented 1 min, closure liver incision, less than commercialized has advantages hemostasis, mild sealant, easy supply by chemical synthesis without inhibition anticoagulants, minimize bacterial infection immediate closure. Therefore, could become new-type match surgical for internal bleeding.