High density cultures of embryonic stem cells

• DOI: 10.1002/bit.20650
• Publication Date: 2005-07-25T23:17:31Z
• Authors: Steve K.W. Oh; Wey Jia Fong; Yawen Teo; Heng Liang Tan; Jayanthi Padmanabhan; Angela C.P. Chin; Andre B.H. Choo
• Keywords: Petri dish; Embryoid body; Regenerative Medicine

Embryonic stem cells (ESC) have the unique ability to differentiate into a variety of tissue types. However, realization regenerative medicine will require production large quantities ESC which subsequently be differentiated final phenotype. Thus, we sought develop simple and scaleable bioprocess increase densities achieve this goal. Using mouse embryonic (mESC) as model, by combining automated feeding culture mESC on petriperm dishes, cell were enhanced up 6.4 x 10(6) cells/cm2 compared conventional petri dish only reached 0.2 1.4 cells/cm2. It was found that from all experiments maintained excellent viability, pluripotency, genetic stability after growing for 6 days in cultures with feeding. The expression Oct-4 transcription factor observed cultures, formed embryoid bodies teratomas SCID mice, confirming their karyotype normal. This method stable routine passaging second line shown perform similar manner work represents an important step towards achieving high density ESC.