An epoch‐making application of discharge plasma phenomenon to gene‐transfer

• DOI: 10.1002/bit.20659
• Publication Date: 2005-09-17T03:46:35Z
• Authors: Yasuhiro Ogawa; Noriyuki Morikawa; Akiko Ohkubo‐Suzuki; Sousuke Miyoshi; Hiroyuki Arakawa; Yasuhiro Kita; Shintaro Nishimura
• Keywords: Gene transfer; HeLa

Abstract We discovered an epoch‐making gene transfer method utilizing discharge plasma. Although electroporation is commonly used in present experiments, it cannot genes into primary cells sufficiently. The atmospheric pressure plasma employed this study was originally for surface treatment of non‐biological materials. hypothesized that could provide a suitable effect on the target and applied to various types cells. technology succeeded efficient green fluorescence protein (GFP) plasmid post‐mitotic neuronal obtained from cerebral cortices rats, which with conventional equipment sufficiently, as were attached. After transfection rat pheochromocytoma PC12 GFP by treatment, retained their function, is, nerve growth factor‐induced differentiation. Furthermore, also applicable other cell lines such HeLa Chinese hamster lung (CHL) adherent lines, Jurkat suspended line, another type cell, human umbilical vein endothelial (HUVEC). In conclusion, efficiently transfers genes. Moreover, able universally function maintained. © 2005 Wiley Periodicals, Inc.